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Charles Estienne (; 1504–1564), known as Carolus Stephanus in Latin and Charles Stephens in English, was an early exponent of the science of anatomy in France. Charles was a younger brother of Robert Estienne, the famous printer, and son to Henri, who Latinized the family name as Stephanus. He married Geneviève de Berly.
After the usual humanistic training he studied medicine, and took his doctor's degree at Paris. He was for a time tutor to Jean-Antoine de Baïf, the future poet. It is uncertain whether he taught publicly. His career was interrupted by the oppressive in which their religious opinions involved the family.
Éstienne, though from a family whose classical taste was their principal glory, did not betray the same servile imitation of the anatomy as his contemporary, Jacques Dubois. He appears to have been the first to detect valves in the orifice of the , though his description was vague. He was ignorant, however, of the researches of the Italy anatomists; and his description of the Human brain is inferior to that given sixty years before by Alessandro Achillini. His comparison of the cerebral cavities to the human ear has persuaded F. Portal that he knew the inferior cornua, the hippocampus and its prolongations; but this is no reason for giving him that honour to the detriment of the reputation of Achillini, to whom, so far as historical testimony goes, the first knowledge of this fact is due.
The researches of Éstienne into the structure of the nervous system are, however, neither useless nor inglorious; and the circumstance of demonstrating a canal through the entire length of the spinal cord, which had neither been suspected by contemporaries nor noticed by successors till Jean-Baptiste de Sénac (1693–1770) made it known, is sufficient to place him high in the rank of anatomical discoverers.
In 1551, when Robert Estienne left Paris for Geneva, Charles, who had remained a Catholic, took charge of his printing establishment, and in the same year was appointed king's printer. In 1561 he became bankrupt, and he is said to have died in a debtors' prison.
His principal works are:
His daughter Nicole Estienne became a prolific poet.
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